Sponsored Seminars

Agilent Lunch Seminars
Location: Room 203, North Building, Metro Toronto Convention Centre

Tuesday, August 23

12:30 pm – 1:30 pm

Presenter Philip Britz-Mckibbin, Associate Professor, Cystic Fibrosis Canada Researcher

Title of Talk: Improving Sample Throughput, Costs and Sata Comparability in Mass Spectrometry using Multiplexed Separations

Abstract: Separation science plays a key role in enhancing the selectivity, sensitivity and robustness of mass spectrometry (MS)-based metabolomic studies. However, low sample throughput and complicated data processing remain major bottlenecks to biomarker discovery when performing untargeted metabolite profiling. Herein, we introduce multi segment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS) as a multiplexed separation platform that takes advantage of customized serial injections to enhance sample throughput and data fidelity with quality assurance. MSI-CE-MS offers a cost effective approach for greatly expanding the productivity of MS-based chemical analyses while offering an accelerating data workflow for biomarker discovery in metabolomics.

For research use only. Not for use in diagnostic procedures.


Presenter: Christine Des Rosiers Ph.D., Director, Metabolomics Platform, Montreal Heart Institute (MHI), Professor, Department of Nutrition & Biochemistry, Université de Montréal

Title of Talk: Using metabolomics to translate genetic discoveries in personalized medicine approach: Lessons from the iGenoMed Consortium in inflammatory bowel disease 

Abstract: Metabolomics - the most recent addition to the “omics” disciplines - offer a means to measure thousands of low molecular weight compounds from any cell, tissue or body fluid. This provides a global view of alterations in metabolic pathways induced by a given perturbation, whether resulting from a gene mutation or disease onset. Recent developments in technologies have enabled the application of metabolomics in the clinics in a high-through-put manner together with other omics. There are, however, numerous challenges and issues, which need to be taken into account for the successful application of metabolomics to disease biomarker discovery particularly in the setting of large cohort studies integrating other omics data. This presentation will illustrate how we are tackling these challenges in the context of a multidisciplinary personalized medicine project on inflammatory bowel diseases (IDB) as part of the iGenoMed Consortium (http://www.medgeni.org/node/1), which exploits known IBD genetic risk factors to develop a tests for response to therapy.

For Research Use Only. Not for use in diagnostic procedures.


Bruker Lunch Seminars
Location: Room 206A-D, North Building, Metro Toronto Convention Centre

Monday, August 22

12:30 pm – 1:30 pm

Presenter: Jeffrey N. Agar, Associate Professor and Barnett Institute Fellow, Depts. of Chemistry and Pharm. Sci., Northeastern University

Title: Tracking the Dark Metabolome with a Novel Isotopic Fine Structure Enabled Metabolic Labeling Strategy

Tuesday, August 23

12:30 pm – 1:30 pm

Presenter: Mel Park, Bruker Daltonics, Director of Research

Title of Talk: Ion Mobility Mass Spectrometry Coming of Age

Presenter: Dr. Sven Meyer, Senior R&D Scientist OMIC’s Solutions

Title of Talk: A New Dimension to QTOF Technology in the Analytical Lab

To attend the Bruker seminars, please click here to register.


LECO Lunch Seminar
Location: Room 206EF, North Building, Metro Toronto Convention Centre

Tuesday, August 23

12:30 pm – 1:30 pm

Presenter: Susan D. Richardson, Arthur Sease Williams Professor of Chemistry, University of South Carolina

Title of Talk: A Novel GC-TOF-MS:  Quantifying Priority Unregulated Disinfection By-Products with the Sensitivity of SIM while acquiring Full Range Mass Spectra for Unknown Identification


Thermo Scientific Lunch Seminars
Location: Room 205, North Building, Metro Toronto Convention Centre

Monday, August 22

12:30 pm – 1:30 pm

Presenter: TBA

Title of Talk: Protein Structure Analysis with Cross-Linking and Top Down MS

Abstract: Chemical cross-linking combined with mass spectrometry (CX-MS) and Top Down mass spectrometry are powerful methods to probe the structure of proteins, complexes and interactions. The high resolution and mass accuracy of Orbitrap technology, in combination with enhanced fragmentation modes (e.g., HCD, ETD, EtHCD) and MSn modalities, allow completely unique and effective methods to probe protein structure. New reagents and software facilitate sample preparation and data interpretation to make these capabilities available to all labs. This seminar will feature the latest work on protein structure elucidation, protein characterization and protein-protein interaction networks.


Tuesday, August 23

12:30 pm – 1:30 pm

Presenter: TBA

Title of Talk: Complete Characterization of Biologics: mAbs and ADCs

Abstract: Peptide mapping, subunit top/middle down analysis, and intact native and denatured analysis: The new Thermo Scientific™ Q Exactive™ BioPharma solution can do it all. Use the high resolution and mass accuracy of Orbitrap technology to generate exemplary data and Thermo Scientific™ BioPharma Finder™ software to analyze sequence variance, sites of modification, carbohydrate structure, etc. Verify these findings at the subunit level with top/middle down sequence analysis. Get a full picture of biologic heterogeneity, including glycosylation, linkers, drug conjugates, etc., with denatured and native intact analysis. This seminar will introduce the Q Exactive BioPharma solution and showcase examples of comprehensive characterization of biologic drugs.


Wednesday, August 24

12:30 pm – 1:30 pm

Presenter: TBA

Title of Talk: Advances in Accurate, High-Throughput Quantitative Proteomics

Abstract: The ability to perform accurate protein quantification at low levels lets scientists unravel the complexity of protein interactions and track protein abundance changes in a wide variety of samples. When combined with multiplexing capabilities, quantitative proteomics provides a deep and comprehensive understanding of the molecular mechanisms underlying biological processes and disease states. Thermo Scientific™ TMT™ isobaric mass tag labeling combined with Orbitrap high resolution accurate mass (HRAM) mass spectrometry (MS) enable greater multiplexing capacity, resulting in increased depth of quantitative proteomic analysis across larger numbers of samples. The improved sensitivity and accuracy achieved with Orbitrap HRAM MS and synchronous precursor selection (SPS)-based MS3 technology on the Thermo Scientific™ Orbitrap Tribrid™ MS systems provide a unique, unmatched capability to accurately measure the most subtle changes in low-abundance proteins. In this seminar, we will discuss multiplexed quantitative proteomics and present real-world applications from leading research laboratories.


Waters Lunch Seminars
Location: Room 202, North Building, Metro Toronto Convention Centre

Monday, August 22

12:30 pm – 1:30 pm

Presenter: David Heywood, Waters Corporation

Title of Talk: Making large scale Omics workflows routine

Abstract: The analysis of complex biological samples in support of “Omics” style workflows challenges current analytical systems. In this seminar we will discuss the role of ion mobility and informatics which have been developed to increase coverage and streamline biomarker identification in a robust, reproducible and accessible manner.

To attend this seminar, please click here to register.


Tuesday, August 23

12:30 pm – 1:30 pm

Presenter: Xavier Ortiz Almirall, Ministry of the Environment and Climate Change

Title of Talk: Towards an automated untargeted method for microcystins analysis using two dimensional liquid chromatography and ion mobility/quadrupole time of flight mass spectrometry

Abstract: Microcystins are cyclic heptatpeptide hepatotoxins produced by certain species of cyanobacteria (blue-green algae) found in freshwater environments. These secondary metabolites are toxic to higher organisms, causing human sickness or even death in some cases. Even though only one particular variant is currently regulated under the Ontario Safe Drinking Water Act (microcystin-LR, 1.5 µg/L) and there is only a handful of microcystin standards available in the market, over 90 different microcystins variants have been reported to date. For this reason, it is important to develop non-targeted methods for the analysis of these compounds. The present study describes an automated method for the analysis of microcystins by two dimensional liquid chromatography/quadrupole time of flight mass spectrometry (2D-LC/QTOF-MS) using a Waters Acquity I-Class and Waters Xevo G2-XS. Moreover, uncompleted chromatographic separation of all microcystins variants was further achieved by ion mobility mass spectrometry.

An automated method for microcystins extraction and clean up from water samples was developed using 2D-LC in the trap and elute configuration was used: a large volume of water sample (500 µl) was directly injected and trapped in the first column. After that, microcystins were desorbed in reverse flow and injected to the analytical column, prior to mass spectrometry analysis. When the QTOF-MS was operated in high resolution full scan mode (RP ≈ 25.000), the instrument proved to be very sensitive for microcystin-LR (50 fg on column with S/N > 10). Combined with the 2D-LC, the system could detect 100 pg/L of microcistyn-LR in water with S/N>10. Mass accuracy (< 1 ppm) allowed assigning elemental compositions for unknown compounds with confidence. MS/MS mode monitoring the characteristic microcystin ion at m/z=135.0804 was useful to provide quantitative results of targeted compounds in complex samples.

For a more comprehensive characterization of complex algal bloom samples, ion mobility was used to distinguish the different microcystin variants based on their cross-section. This new dimension of separation allowed the identification of some congeners that could not be separated by means of chromatography or mass spectrometry alone. Advanced acquisition methods such as Data Dependant Acquisition (DDA) and Data Independent Acquisition (DIA) were successfully employed to elucidate new microcystin variants in real samples that have not been reported in literature yet.

To attend this seminar, please click here to register.


Wednesday, August 24

12:30 pm – 1:30 pm

Presenter: TBA

Title of Talk: Molecular visualization - current and future perspectives on Mass Spectrometry Imaging

Abstract: In this lunchtime session, Waters will discuss the latest developments in Mass Spectrometry imaging. We will present the data from both our internal and collaborative research programs, and look at the utility of Mass spec imaging across both preclinical and clinical research.

To attend this seminar, please click here to register.


Waters Breakfast Seminars
Location: Waters' Hospitality Suite, Room 715, South Building, Metro Toronto Convention Centre

Tuesday, August 23

7:00 am – 8:00 am

Presenter: Dr. Roger Linington, Simon Fraser University

Title of Talk: Integration of High-Content Screening and Untargeted Metabolomics for Comprehensive Functional Annotation of Natural Product Libraries


 Abstract: Traditional natural products discovery using a combination of live/dead screening followed by iterative bioassay-guided fractionation affords no information about compound structure or mode of action until late in the discovery process. This leads to high rates of rediscovery and low probabilities of finding compounds with unique biological and/or chemical properties. By integrating image-based phenotypic screening in HeLa cells with high-resolution untargeted metabolomics analysis, we have developed a new platform, termed Compound Activity Mapping, which is capable 
 of directly predicting the identities and modes of action of bioactive constituents for any complex natural product extract library. This new tool can be used to rapidly identify novel bioactive constituents and provide predictions of compound modes of action directly from primary 
 screening data.

This approach inverts the natural products discovery process from the existing ‘grind and find’ model to a targeted, hypothesis-driven discovery model where the chemical features and biological function of bioactive metabolites are known early in the screening workflow, and lead compounds can be rationally selected based on biological and/or chemical novelty. Advantages of employing integrated Big Data approaches to natural products discovery will be presented, as well as current challenges and limitations associated with these emerging technologies.

To attend this seminar, please click here to register.


Wednesday, August 24

7:00 am – 8:00 am

Presenter: Patty Sun, Waters Corporation

Title of Talk: ionKey [LC/MS] HT- improvements for LC/MC integration

As microflow LC-MS systems continue to evolve, an increasing number of users are looking into applying microflow technologies to various scientific fields. In order to satisfy the wide-ranged needs and provide flexibility in this growing market, the ionKey/MS HT products are introduced as the expansion of the current ionKey/MS offer by adding a series of new features. During the initial launch of the ionKey/MS HT product family, following iKey separation devices are released: 300 µm ID and 5 cm BEH 130Å PsT C18, 1.7 µm, CSH 130Å PsT C18 CSH, 1.7 µm, and HSS T3 100Å, 1.8 µm.

In this presentation, we will discuss the new features of the ionKey/MS HT product family and the benefits of applying 300 µm ID iKey to certain applications. Sensitivity gain and reduced matrix effects are the well-known benefits of micro-scale LC/MS. However, the throughput of microflow LC/MS systems has been a challenge for laboratories performing routine analyses with large number of samples. The 300 µm ID iKey devices packed with the sub 2.0 µm particles achieve the sensitivity gain, throughput, and UPLC grade separation power by using higher flow rates and shorter cycle times.

To attend this seminar, please click here to register.


Key Dates

  • 2016-01-01: Registration Opens
  • 2016-01-01: Abstract Submissions Start
  • 2016-05-06: Abstract Submissions for Orals Closes (extended)
  • 2016-05-20: Abstract Submissions for Posters Closes (extended)
  • 2016-05-30: Early Registration End
  • 2016-08-20: Short Courses Start
  • 2016-08-21: Conference Starts

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